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Cell Regeneration
Fig. 2 | Cell Regeneration

Fig. 2

From: CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells

Fig. 2

CTCF-K20 is deacetylated by HDAC6. (A) Detection of the levels of FLAG-CTCF and CTCF-K20ac in HEK293T cells treated with TSA (10 μM), NAM (5 mM) or both. Membranes were immunoblotted with the indicated antibodies after Flag co-IP experiments. (B and C) Western blots showing CTCF, CTCF-K20ac, β-ACTIN and FLAG-tagged HDAC levels in HEK293T cells transfected with different HDAC constructs, respectively. (D) Western blots showing HDAC6, CTCF, CTCF-K20ac and β-ACTIN levels in HEK293T cells transfected with the scrambled control shRNA or HDAC6 shRNA. (E) Western blots showing CTCF, CTCF-K20ac, FLAG and β-ACTIN levels in HEK293T cells treated with 10 μM TBSA for 6 h

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