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Fig. 4 | Cell Regeneration

Fig. 4

From: CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells

Fig. 4

CTCF-K20R reduced CTCF binding in differentiated EBs. (A) Differential analysis of CTCF binding in both WT and CTCF-K20R mESCs and differentiated EBs at day 4 after differentiation. Two independent CTCF ChIP-seq experiments were performed for each sample. (B) The average binding strength for different types of CTCF sites using CTCF binding sites in WT EBs on day 4. (C) Motif analysis of CTCF binding sites in both WT and CTCF-K20R EB samples on day 4. The CTCF motif is derived from Homer. (D) Genomic distribution of CTCF binding sites in EB samples on day 4. (E) Scatter plot showing the DEGs located near the sites with decreased CTCF binding. (F) GO analysis of the DEGs shown in Fig. 4E. Green bars represent the top GO terms for the downregulated genes, and red bars represent the top GO terms for the upregulated genes

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