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Cell Regeneration
Fig. 5 | Cell Regeneration

Fig. 5

From: CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells

Fig. 5

Relationship between reduced CTCF binding and chromatin accessibility. (A) Differential analysis of chromatin accessibility in both WT and CTCF-K20R differentiated EBs on day 4. Two independent ATAC-seq experiments were performed for each sample. (B) Heatmaps showing the CTCF binding signal and chromatin accessibility in both WT and CTCF-K20R EB samples on day 4 after differentiation. (C) Genome browser views of CTCF ChIP-seq and ATAC-seq data for selected loci in both WT and CTCF-K20R samples. (D) Scatter plot showing the expression change for genes with reduced CTCF binding in their promoters. (E) Comparison of chromatin accessibility at the promoters of genes shown in Fig. 5D in both WT and CTCF-K20R differentiated EBs on day 4. (F) GSEA analysis for the genes shown in Fig. 5D, which were ranked by log2 fold change comparing CTCF-K20R to WT differentiated EBs on day 4

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