Fig. 4
From: Glioma stem cells and neural stem cells respond differently to BMP4 signaling
BMP4 reduced S100-beta positive, Sox2 positive and DAPI staining in hGSCs, but no significant decrease in Ki67 positive cells. a Representative image of the sphere area of hGSCs immunostained for S100-beta, Ki67, Sox2, and DAPI (nuclei). Control (untreated) and BMP4-treated (20 ng/mL) hGSCs were fixed and stained after 6 days of treatment. b Representative images of (a) at higher magnification (control and BMP-treated [20 ng/mL]). c Representative images of nonsphere area hGSCs immunostained for S100-beta, Ki67, Sox2, and DAPI (nuclei). d Representative images of (c) at higher magnification. e Quantitative analysis of DAPI, Ki67, S100-beta, and Sox2 expression in BMP4-stimulated hGSCs measured using ImageJ. Data are presented as means ± SD. Student’s t-test. ∗p < 0.05