Fig. 2
The effect of strain magnitude, frequency, and timing window of mechanical force on organoid culture. a Imaging of cyclic stretch (0.2 Hz) started on Day 3 at various amplitudes, with 8% strain to be an optimum condition. b, c and d showed the data analysis for strain timing, magnitude, and frequency. The statistical data represent mean ± s.d. (n = 6 organoids derived from three independent experiments for each condition). Student’s t-test: ***P < 0.001. **P < 0.01. *P < 0.05. Scale bar, 100 μm