Fig. 4

Mechanical stretching induces cell proliferation. a Imaging and quantification of IF staining for Ki67 in organoid cultured with 8% cyclic stretching (right panel) or under static condition (left panel), respectively. b Imaging and quantification of IF staining for Mmp7 in organoid cultured with 8% cyclic stretching (right panel) or under static condition (left panel), respectively. c Imaging and quantification of IF staining for Sox9 in organoid cultured with 8% cyclic stretching (right panel) or under static condition (left panel) respectively. n = 6 organoids derived from three independent experiments for each condition. Student’s t-test: ***P < 0.001. **P < 0.01. *P < 0.05. Scale bars, 50 μm