Skip to main content
Cell Regeneration
Fig. 6 | Cell Regeneration

Fig. 6

From: Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal

Fig. 6

Stretching induces the Wnt/β-Catenin pathway. a IF staining of organoid cultivated without R-Spondin1. Organoids were stained with DAPI and immunostained for Sox9 after stretching. b Quantification of organoid size cultivated without R-Spondin1. c Quantification of Sox9+ cell ratio of organoid cultivated without R-Spondin1. d Venn diagram showing the overlap among up-regulated genes (red), down-regulated genes (blue), NO R-Spondin1 up-regulated genes (yellow), NO R-Spondin1 down-regulated genes (green). The significance was evaluated by Fisher’s exact test. e To test the expression level of Lgr5, Ascl2, Sox9, and Olfm4 using qRT-PCR. Scale bar, 50 μm

Back to article page