Fig. 2

Taz promotes the processing of Gli3F into Gli3R in C3H10T1/2 cells. A, B Western analyses in C3H10T1/2 cells transfected with different doses of myc-Taz and followed by culture for 24 hrs. C Western analyses of Gli3F and Gli3R in cytosolic and nuclear fractions of C3H10T1/2 cells transfected with myc-Taz and followed by culture for 24 hrs. D Western analyses in C3H10T1/2 cells transfected with Taz siRNA for 24 hrs and followed by culture for further 24 hrs. E-H Western analyses in C3H10T1/2 cells transfected with myc-Gli3/myc-Gli3R and myc-Taz /Taz siRNA and followed by culture for 24 or 48 hrs. All the western experiments were either duplicated or triplicated and the mean value was present under each band