Fig. 3

Taz drives the phosphorylation of Gli3 by PKA in C3H10T1/2 cells. A Schematic representation of PKA phosphorylation sites on Gli3 and the Gli3 processing into Gli3R. B Western analyses in C3H10T1/2 cells transfected with myc-Taz and followed by culture for 12 hrs. C, D Western analyses in C3H10T1/2 cells transfected with myc-Taz or Taz siRNA in combination with myc-Gli3 (WT) or myc-Gli3 sextuple mutant (MT) and followed by culture for 24 hrs. E-H Western and Gli-luciferase analyses in C3H10T1/2 cells transfected with myc-Taz and cultured for 42 hrs following the treatment with H-89 at 10 μM or transfection with PKAc siRNA. I-L Western and Gli-luciferase analyses in C3H10T1/2 cells treated with forskolin at 20 μM or transfected with PKAc following the transfection with Taz siRNA and culture for 42 hrs. Western analyses were either duplicated or triplicated and the mean value was present under the bands. Error bars, SD; *^,+ p < 0.05, ** p < 0.01, n = 3