Fig. 5
From: Hippo signaling activates hedgehog signaling by Taz-driven Gli3 processing
Activation of Hippo signaling promotes Hh signaling. A Western analyses of Gli3F, Gli3R, and Taz in C3H10T1/2 cells at the density of 1 × 103 cells/cm2 (low density, L) and 5 × 105 cells/cm2 (high density, H). B-E Gli-luciferase and western analyses in C3H10T1/2 cells transfected with Lats1 or in CRISPR/Cas9-mediated Lats1-knockout C3H10T1/2 cells (sgLats1) and further cultured with or without N-Shh at 100 ng/ml for 42 hrs. F-H Western analyses in primary MEFs from Taz+/− and Taz−/− embryos at E14.5 or in NIH3T3 cells expressing myc-Taz or Taz siRNA. I-L Western and immunohistochemistry analyses in kidneys from embryos with the indicated genotypes at E16.5. Western and immunostaining analyses were either duplicated or triplicated and the mean value was present under the bands. Error bars, SD; *p < 0.05, **,++ p < 0.01, n = 3. Scale bars, 50 μm