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Fig. 6 | Cell Regeneration

Fig. 6

From: Hippo signaling activates hedgehog signaling by Taz-driven Gli3 processing

Fig. 6

Taz interacts genetically with Shh in the embryonic limb patterning. A Whole-mount in situ hybridization for Taz(left) and Shh(right) in the forelimb from E10.5 mouse embryos. B Western analyses for Taz in anterior and posterior halves of limb buds from embryos at E10.5. C Western analyses in pooled limb buds from embryos with the indicated genotypes at E10.5. The values derive from the normalized immunoreactive bands (n = 5). D Wetern analyses for Gli3F, Gli3R, and Taz in pooled anterior and posterior halves of limb buds with the indicated genotypes at E10.5. E qRT-PCR analyses in pooled limb buds with the indicated genotypes at E10.5. F Whole-mount in situ hybridization of the E10.5 hindlimbs from ShhCre−/−;Taz+/+ (Cre−/−;Taz+/+), ShhCre−/−;Taz−/− (Cre−/−;Taz−/−), ShhCre+/+;Taz+/+ (Cre+/+;Taz+/+) and ShhCre+/+;Taz−/− (Cre+/+;Taz−/−) embryos. G Representative E16.5 forelimbs and hindlimbs from embryos with the indicated genotypes. The representative results from three to four independent experiments were shown and the mean value was present under the bands. Red arrows denote the defects in limbs. For whole-mount in situ hybridization, ventral view for all limb buds, anterior to the left and posterior to the right, Scale bars, 50 μm. Error bars, SD; **,++ p < 0.01, n = 3

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