Fig. 6

Regulons activated during the initiation of antler generation and regeneration. A Venn diagram to show the activated regulons during differentiation from DoAP to AcAP, and those from DoPP to AcPP. The 61 shared regulons of the DoAP and AcAP (green and orange background), and the 32 shared regulons of the DoPP and AcPP (blue and orange background), and the 18 shared regulons of antler generation, and regeneration are in orange background. B Heatmap plot to show the activity of AR regulon in the eight-cell types of the DoAP (upper panel) and AcAP (lower panel). The activity of AR regulon was calculated using the area under the curve (AUC). Note that activity of AR regulon was significantly higher in THY1⁺ cells and progenitor cells. AR regulon in the AcAP includes 27 genes (red background) and in the DoAP includes 12 genes (pink background). RXFP2 and AR are in orange. Violin plots to show the expression levels of AR and RXFP2 in AR regulon in the DoAP (C) and in the AcAP (D). E Expression levels of RXFP2 mRNA in the AcAP and DoAP using qRT-PCR. Value: mean ± S.E.M. n=3. F Influence of DHT on the expression levels of RXFP2 mRNA in the AP cells based on qRT-PCR analysis. Different concentrations (2, 5, 10 nM) of DHT were applied for 24 h. Value: mean ± S.E.M. n=3, NC: Normal control