Table 2 Comparison of technologies and their applications in studies of neurite regeneration and repair
From: Molecular mechanisms of neurite regeneration and repair: insights from C. elegans and Drosophila
Technologies | Application | Applicable model animal | Advantages | Disadvantages | References |
|---|---|---|---|---|---|
Femtosecond laser surgery | Vaporizing targeted single axon or dendrite branch | C. elegans; Drosophila | Quick, and precise | Relatively expensive | Galbraith and Terasaki 2003; Sugimura et al. 2003; Yanik et al. 2004 |
Microinjection needle surgery | Mechanical truncation of single dendrite branch | C. elegans | Low cost and convenient | Requires intense training | Zhao et al. 2021 |
Forceps pinched surgery | Mechanical crushing of neuronal axons | Drosophila | Quick, low cost | Technically challenging | Xiong et al. 2010 |
Mechanical stress induced spontaneous axonal break | unc-70/β-spectrin mutants are capable of regenerating and repairing axonal breaks | C. elegans | Convenient | Only applies to specific neurons | Nix et al. 2014 |