Table 2 The heterogeneity of adipose precursors revealed by scRNA-seq analysis
From: Characteristic and fate determination of adipose precursors during adipose tissue remodeling
Author | Species | Sample | Pre-processing | Population | Markers | Definition or function |
|---|---|---|---|---|---|---|
Merrick et al (Merrick et al. 2019) | Mouse | IngWAT | CD45- | Group 1 | Dpp4+ , Wnt2+ , Bmp7+ , Pi16+  | Interstitial progenitors (produce ICAM1+ and CD142+ preadipocytes) |
Group 2 | Icam1+ , Pref1+ , Pparg+ , Fabp4+ , Cd36+  | Committed preadipocytes | ||||
Group 3 | Cd142+ , Clec11a+ , Fmo2+  | Another preadipocytes | ||||
Human obese | sWAT | CD45- | Group 1 | DPP4+ , CD55+ , WNT2+  | Comparable to murine group 1 cells | |
Group 2 | ICAM1+ , PPARG+ , GGT5+  | Comparable to murine group 2 cells and group 3 cells | ||||
Vijay et al (Vijay et al. 2020) | Human obese | sWAT | CD45- CD34+  CD31- | SP1 and SP3 | MGP+ , APOD+ , CXCL14+ , WISP2+  | Preadipocytes/adipose stem cells |
SP2 | APOE+ , FABP4+ , CEBPB+ , CD36+  | More mature adipocytes progenitor cells | ||||
SP4 | COL3A1+ , COL6A3+ , COL1A1+ , COL6A1+  | Fibrosis and extra cellular matrix accumulation | ||||
vWAT | CD45- CD34+  CD31- | VP4 | APOD+ , CXCL14+ , DPT+ , GPX3+ , MGP+ , EIF1+ , C1S+ and ADH1B+  | Similar to SP1 and SP3 | ||
VP5 | MFAP5+ , S100A4+  | Adipose tissue remodeling; inhibition of adipogenesis | ||||
Oguri et al (Oguri et al. 2020) | Mouse | iBAT, IngWAT, EpiWAT | Lin- stromal cells | Types I | Pdgfra+ , Akt2+ , Wnt4+  | Not mentioned |
Type II | Pdgfrb+ , Cspg4+ , Ednrb+  | An endothelial signature | ||||
Type III | Cd81+ , Pdgfra+ , Sm22+ , Acta2+  | From IngWAT; contribute to de novo beige adipogenesis | ||||
Types IV | Pdgfra+ , Mmp3+  | Not mentioned | ||||
Types V | Pdgfra+ , Atf6+ , Acot2+  | Not mentioned | ||||
Emont et al (Emont et al. 2022) | Mouse | IngWAT and EpiWAT of mice fed ND or HFD | stromal vascular fractions | mASPC1 | Pdgfra+ , Pde11a+  | Not mentioned |
mASPC2 | Pdgfra+ , Aldh1a3+  | Early progenitor cells; more abundant in IngWAT than EpiWAT; differentiate into mASPC6 upon HFD | ||||
mASPC3 | Pdgfra+ , Mgp+  | Not mentioned | ||||
mASPC4 | Pdgfra+ , Epha3+  | Not mentioned | ||||
mASPC5 | Pdgfra+ , Tenm3+  | More prevalent in EpiWAT versus IngWAT | ||||
mASPC6 | Pdgfra+ , Frem1+  | More prevalent in EpiWAT versus IngWAT, increase markedly in response to HFD in PG | ||||
Human | sWAT and vWAT | hASPC1 | PDGFRA+ , CEBPD+  | More prevalent in sWAT than vWAT | ||
hASPC2 | PDGFRA+ , ALDH1A3+  | Not mentioned | ||||
hASPC3 | PDGFRA+ , FGF10+  | More abundant in vWAT | ||||
hASPC4 | PDGFRA+ , EPHA3+  | More abundant in sWAT than vWAT; increases in sWAT in subjects with higher BMI | ||||
hASPC5 | PDGFRA+ , SGCZ+  | More abundant in sWAT than vWAT; increases in sWAT in subjects with higher BMI | ||||
hASPC6 | PDGFRA+ , PDE4D+  | More prevalent in vWAT | ||||
Burl et al (Burl et al. 2018) | Mouse | IngWAT and EpiWAT of control and CL316243 (β3-adrenoceptor agonists)-treated mice | Lin- stromal cells | ASC1 | Pdgfra+ , Sca1+ , Icam1+ , Col4a2+  | Adipocyte stem cell group 1 |
ASC2 | Pdgfra+ , Sca1+ , Pi16+ , Dpp4+  | Adipocyte stem cell group 2; more prevalent in IngWAT | ||||
Diff.ASC | Plin1+ , Car3+ , Cebpa+  | Differentiating adipocyte stem cells; CL316243-induced clusters in EpiWAT | ||||
Pro.ASC | Genes that positively regulate cell cycle | Proliferating adipocyte stem cells; CL316243-induced new clusters in IngWAT and EpiWAT | ||||
Hepler et al (Hepler et al. 2018) | Mouse | EpiWAT | PDGFRβ+  | APCs | Ly6c-, Cd9- | Adipocyte precursor cells |
FIPs | Ly6c+ , Cd9+  | Fibro-inflammatory progenitors | ||||
MLCs | Ly6c+ , Cd9- | Mesothelial-like cells | ||||
Schwalie et al (Schwalie et al. 2018) | Mouse | IngWAT | Lin- CD29+  CD34+  Sca1+  | G1/G4 (P1) | Cd55+ , Il13ra+  | Adipose stem cells |
G2 (P2) | Vap1+ , Adam12+  | Preadipocytes | ||||
G3 (P3) | Cd142+ , Abcg1+  | Adipogenesis-regulatory cells; inhibit adipogenesis | ||||
Cho et al (Cho et al. 2019) | Mouse | EpiWAT of mice fed ND or HFD | Sca1+  CD45- CD31- Ter119- | Cluster1 | CD55high/CD81low | More primitive, undifferentiated ASCs |
Cluster5 | CD55high/CD81high | More primitive, undifferentiated ASCs | ||||
Cluster2 | CD55low/CD9low−mid/CD81low | Committed preadipocytes | ||||
Cluster4 | CD55low/CD9low−mid/CD81high | Committed preadipocytes | ||||
Cluster6 | CD55low/CD9high | Committed preadipocytes | ||||
Nahmgoong et al (Nahmgoong et al. 2022) | Mouse | IngWAT and EpiWAT of mice fed ND or HFD | Lin- (CD31- and CD45-) stromal vascular fractions | EN1 and IN1–IN4 | Dpp4+  | Multipotent ASCs expressing BMPs |
EN2–EN3 and IN5 | Dpp4+ , Icam1+ , Cebpd+  | Early committed preadipocytes | ||||
EN4 and IN6–IN7 | Icam1+ , Fabp4+  | Late committed preadipocytes |