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Fig. 3 | Cell Regeneration

Fig. 3

From: Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

Fig. 3

Functional characterization of hSCNPCs direct differentiated posterior spinal motor neurons. A Representative recording traces of hSCNPCs-derived spinal motor neurons from day 6 to day 42 in response to step current injection. B Percentages of hSCNPCs-derived spinal motor neurons from day 6 to day 42 with AP and no AP. N numbers of technical replicates were shown in figures from 3 independent experiments. Data are represented as mean ± SD. C Quantification of AP amplitude of hSCNPCs-derived spinal motor neurons from day 6 to day 42. N numbers of technical replicates were shown in figures from 3 independent experiments. Data are represented as mean ± SD. D Quantification of AP threshold during hSCNPCs spinal motor neurons differentiation from day 6 to day 42. N numbers of technical replicates were shown in figures from 3 independent experiments. Data are represented as mean ± SD. E Quantification of resting membrane potential (RMP) of hSCNPCs-derived spinal motor neurons from day 6 to day 42. N numbers of technical replicates were shown in figures from 3 independent experiments. Data are represented as mean ± SD. F Quantification of input resistance (Rin) of hSCNPCs-derived spinal motor neurons decreased over the differentiation process. N numbers of technical replicates were shown in figures from 3 independent experiments. Data are represented as mean ± SD. G Comparison of F-I curves from hSCNPCs-derived spinal motor neurons at day 6, day 12, day 18, day 24, day30 and day 42. Data were collected from 3 independent experiments. Data are represented as mean ± SD. H Spatial distribution maps of active spikes and the quantification results during spinal motor neuron differentiation from day 6 to day 42. n = 3 independent experiments. Data are represented as mean ± SD. I Representative mean firing rate and the quantification results during spinal motor neuron differentiation from day 6 to day 42. n = 3 independent experiments. Data are represented as mean ± SD. J Representative axon tracking maps during spinal motor neuron differentiation from day 6 to day 42. K Quantification results of axon tracking during spinal motor neuron differentiation from day 6 to day 42. Data were collected from 3 independent experiments. Data are represented as mean ± SD. L Network analysis during spinal motor neuron differentiation from day 6 to day 42 and cortical neuron at day 18 as a positive control. M Immunostaining of pre- (SYP, Synaptophysin) and post-synaptic (PSD95) markers of hSCNPCs-MNs and cortical neurons at day 18. Scale bar, 50 μm

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