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Fig. 6 | Cell Regeneration

Fig. 6

From: Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

Fig. 6

Co-culture of hSCNPCs-derived spinal motor neuron spheroids and C2C12-derived muscle fibers to form neuromuscular junctions in vitro. A Representative bright-field images of the differentiated spinal motor neuron spheroids based on differential co-culture medium compositions. Scale bar, 100 μm. B Representative bright-field images of the differentiated muscle fibers based on differential co-culture medium compositions. Scale bar, 100 μm. C Fold changes of mature motor neuron marker genes (NEUN, ChAT and VAChT) expression on differential co-culture medium. Data were collected from 2 independent experiments. Data are represented as mean ± SD. D Fold changes of AChR markers (musk, chrng and chrne) genes expression on differential co-culture medium. Data were collected from 2 independent experiments. Data are represented as mean ± SD. E Schematic illustration of co-culture model. F Immunofluorescent images demonstrating motor neurons extending patterns when co-cultured with muscle fibers. Scale bar, 50 μm. G Immunofluorescent staining of neuronal marker (TUJ1), mature muscle marker (MyHC) and AChR marker (α-BTX) on co-cultured cells. Scale bar, 50 μm

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