TAD reorganization: a new mechanism for cell fate determination

© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. The Creative Commons Public Domain Dedication waiver (http:// creat iveco mmons. org/ publi cdoma in/ zero/1. 0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Revealing how master transcription factors promote cell fate transitions through controlling TAD reorganization, in the recent issue of Cell Stem Cell, Wang et al. (2021) illustrate the phase separation of OCT4 controls TAD reorganization for cell fate transitions. The eukaryotic three-dimensional (3D) genome is organized in a hierarchical order, mainly comprising compartments, topological-associated domains (TADs), and chromatin loops (Dixon et al., 2012; LiebermanAiden et al., 2009; Rao et al., 2014). 3D chromatin architectures are drastically altered during cell fate transitions, which plays an important role for these processes. Unlike compartments and chromatin loops, TADs are usually considered to be stable among different cell types and species (Dixon et al., 2012). However, recent studies have reported the loss of TADs during pluripotent stem cell (PSC) differentiation (Bonev et al., 2017; Zhang et al., 2019), indicating that TADs are likely to reorganize. Therefore, it is significant to clarify the role of TAD reorganization in regulating cell fate transitions, and the mechanism by which master transcription factors regulate TAD reorganization. The authors started their journey by investigating whether TAD reorganization contributes to cell fate transitions. They employed two elegant methods to manipulate TAD reorganization. The first is chemicaldependent artificial looping. In this method, two genome loci belonging to adjacent TADs were targeted by dCas9ABI or dCas9-PYL1, respectively. These two dCas9 proteins can be linked together with the small molecular chemical abscisic acid (ABA), and consequently looping of the targeted loci would be established upon ABA addition (Morgan et al., 2017). This looping may shorten the distance of two TADs, which may induce TAD merging. The second is boundary deletion via a CRISPR/Cas9 genome editing tool. For both methods, the two TADs were successfully merged as evidenced by Hi-C heatmap and increased insulation score. Importantly, TAD reorganization, induced by both methods, enhanced reprogramming efficiency, demonstrating that TAD reorganization contributes to cell fate transitions (Fig. 1A). The authors then asked whether master transcription factor OCT4 directly regulates TAD reorganization. They performed OCT4 HiChIP to identify OCT4-mediated chromatin loops (OCT4 loops) during reprogramming, and found that the dynamic of OCT4 loops can induce TAD reorganization by regulating CTCF binding at TAD boundaries. Next, they performed protein immunoprecipitation followed by mass spectrometry (IP-MS) to identify the interacting proteins of OCT4. They found OCT4 interacts with the key structuring factors, such as CTCF, YY1 and cohesin proteins, and co-enriches with these factors at OCT4 loop anchors. When acute deletion of OCT4, the enrichment of the structuring factors at loop anchors is decreased and considerable chromatin loops disappear. These results demonstrate that OCT4 is required for structuring factors to form chromatin loops, which induces TAD reorganization by regulating CTCF binding at TAD boundaries. This suggests that OCT4 directly controls TAD reorganization. The authors then investigated whether phase separation of master transcription factors promotes cell fate transitions. First, they showed that OCT4 forms phaseseparated condensates with liquid-like behavior during reprogramming. Second, to evaluate the role of OCT4 phase separation in regulating reprogramming, Open Access

Revealing how master transcription factors promote cell fate transitions through controlling TAD reorganization, in the recent issue of Cell Stem Cell, Wang et al. (2021) illustrate the phase separation of OCT4 controls TAD reorganization for cell fate transitions.
The eukaryotic three-dimensional (3D) genome is organized in a hierarchical order, mainly comprising compartments, topological-associated domains (TADs), and chromatin loops (Dixon et al., 2012;Lieberman-Aiden et al., 2009;Rao et al., 2014). 3D chromatin architectures are drastically altered during cell fate transitions, which plays an important role for these processes. Unlike compartments and chromatin loops, TADs are usually considered to be stable among different cell types and species (Dixon et al., 2012). However, recent studies have reported the loss of TADs during pluripotent stem cell (PSC) differentiation (Bonev et al., 2017;Zhang et al., 2019), indicating that TADs are likely to reorganize. Therefore, it is significant to clarify the role of TAD reorganization in regulating cell fate transitions, and the mechanism by which master transcription factors regulate TAD reorganization.
The authors started their journey by investigating whether TAD reorganization contributes to cell fate transitions. They employed two elegant methods to manipulate TAD reorganization. The first is chemicaldependent artificial looping. In this method, two genome loci belonging to adjacent TADs were targeted by dCas9-ABI or dCas9-PYL1, respectively. These two dCas9 proteins can be linked together with the small molecular chemical abscisic acid (ABA), and consequently looping of the targeted loci would be established upon ABA addition (Morgan et al., 2017). This looping may shorten the distance of two TADs, which may induce TAD merging. The second is boundary deletion via a CRISPR/Cas9 genome editing tool. For both methods, the two TADs were successfully merged as evidenced by Hi-C heatmap and increased insulation score. Importantly, TAD reorganization, induced by both methods, enhanced reprogramming efficiency, demonstrating that TAD reorganization contributes to cell fate transitions (Fig. 1A).
The authors then asked whether master transcription factor OCT4 directly regulates TAD reorganization. They performed OCT4 HiChIP to identify OCT4-mediated chromatin loops (OCT4 loops) during reprogramming, and found that the dynamic of OCT4 loops can induce TAD reorganization by regulating CTCF binding at TAD boundaries. Next, they performed protein immunoprecipitation followed by mass spectrometry (IP-MS) to identify the interacting proteins of OCT4. They found OCT4 interacts with the key structuring factors, such as CTCF, YY1 and cohesin proteins, and co-enriches with these factors at OCT4 loop anchors. When acute deletion of OCT4, the enrichment of the structuring factors at loop anchors is decreased and considerable chromatin loops disappear. These results demonstrate that OCT4 is required for structuring factors to form chromatin loops, which induces TAD reorganization by regulating CTCF binding at TAD boundaries. This suggests that OCT4 directly controls TAD reorganization.
The authors then investigated whether phase separation of master transcription factors promotes cell fate transitions. First, they showed that OCT4 forms phaseseparated condensates with liquid-like behavior during reprogramming. Second, to evaluate the role of OCT4 phase separation in regulating reprogramming,

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*Correspondence: songyanz@mail.sysu.edu.cn School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China they generated two OCT4 mutants predicted to disrupt OCT4 phase separation, one with acidic residues to alanine mutations in the intrinsically disordered regions (IDRs) (Boija et al., 2018), and the other with deleted IDR C-terminus (Fig. 1B). Both mutants can effectively disrupt OCT4 phase separation, which attenuates reprogramming. Third, to further verify the role of OCT4 phase separation in cell fate determination, the disabled phase-separated capacity of OCT4 mutants is rescued by fusing an IDR of FUS protein, which is a well-known region to drive liquid-like phase separation (Patel et al., 2015). Interestingly, rescue of OCT4 phase separation restores reprogramming (Fig. 1C), supporting the model that manipulation of phase separation can change cell fate transitions.
Finally, the authors asked the key question whether phase separation regulates TAD reorganization. To answer this question, the authors compared the effects of wildtype and various mutant forms of OCT4 on the alteration of TAD patterns during reprogramming. WT OCT4 induced significantly boundary change compare to negative controls, whereas OCT4 mutants only led to modest boundary change. This indicates that OCT4 can induce TAD reorganization in a phase separationdependent manner. Importantly, the TAD patterns of FUS rescued-OCT4 mutants are similar to those of WT OCT4 but not IDR OCT4 mutants, demonstrating that rescue of OCT4 phase separation can restore TAD reorganization (Fig. 1B). These results for the first time to demonstrate the causal relationship between phase separation and higher-order 3D genome architectures. OCT4 is phase separated during reprogramming. IDR mutation disrupts OCT4 phase separation, which can be rescued by fusing the IDR of FUS behind OCT4 mutant. OCT4 mutants attenuate TAD reorganization, which can be restored by IDR fusion. (C) OCT4 mutants reduce reprogramming efficiency, which can be recovered by IDR fusion