Table 2 Differentiation media of human GI organoids
From: Generation of 3D human gastrointestinal organoids: principle and applications
Organ | Source of organoid | Difference from expansion medium | Differentiated cells | References |
|---|---|---|---|---|
Stomach | Corpus | +Nicotinamide/−Wnt3a | Gland lineage cells/pit lineage cells | (Bartfeld et al., 2015) |
|  | Corpus and antrum | -Wnt3a, −R-spondin1 | MUC5AC-producing pit mucous cells | (Schlaermann et al., 2016) |
Intestine | Small intestine and colon | -Wnt3a, −Nicotinamide, −SB202190 | Goblet cells and enteroendocrine cells | (Sato et al., 2011) |
Colon | -Wnt3a, −Nicotinamide, −SB202190, −PGE2, +DAPTa | Enterocytes, goblet cells and enteroendocrine cells | (Jung et al., 2011) |