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Table 2 Differentiation media of human GI organoids

From: Generation of 3D human gastrointestinal organoids: principle and applications

Organ Source of organoid Difference from expansion medium Differentiated cells References
Stomach Corpus +Nicotinamide/−Wnt3a Gland lineage cells/pit lineage cells (Bartfeld et al., 2015)
  Corpus and antrum -Wnt3a, −R-spondin1 MUC5AC-producing pit mucous cells (Schlaermann et al., 2016)
Intestine Small intestine and colon -Wnt3a, −Nicotinamide, −SB202190 Goblet cells and enteroendocrine cells (Sato et al., 2011)
Colon -Wnt3a, −Nicotinamide, −SB202190, −PGE2, +DAPTa Enterocytes, goblet cells and enteroendocrine cells (Jung et al., 2011)
  1. a10 μM gamma-secretase inhibitor DAPT is added to inhibit Notch signaling