Articles

Protein quality control of cell stemness

Protein quality control (PQC) systems play essential roles in the recognition, refolding and clearance of aberrant proteins, thus ensuring cellular protein homeostasis, or proteostasis. Especially, continued p...

Authors: Pengze Yan, Jie Ren, Weiqi Zhang, Jing Qu and Guang-Hui Liu

Organoid based personalized medicine: from bench to bedside

Three-dimensional cultured organoids have become a powerful in vitro research tool that preserves genetic, phenotypic and behavioral trait of in vivo organs, which can be established from both pluripotent stem ce...

Authors: Yaqi Li, Peiyuan Tang, Sanjun Cai, Junjie Peng and Guoqiang Hua

Combined application of CRISPR-Cas and stem cells for clinical and basic research

Authors: Meng Yan and Jinsong Li

Immunomodulatory and Antioxidative potentials of adipose-derived Mesenchymal stem cells isolated from breast versus abdominal tissue: a comparative study

Adipose-derived stem cells (ASCs) are considered ideal candidates for both research and cellular therapy due to ease of access, large yield, feasibility, and efficacy in preclinical and clinical studies. Unlik...

Authors: Nourhan Abu-Shahba, Marwa Mahmoud, Mazen Abdel-Rasheed, Yasmine Darwish, Ahmad AbdelKhaliq, Eman Mohammed, Mahmoud ElHefnawi and Osama Azmy

Positive feedback between retinoic acid and 2-phospho-L-ascorbic acid trisodium salt during somatic cell reprogramming

Retinoic acid (RA) and 2-phospho-L-ascorbic acid trisodium salt (AscPNa) promote the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells. In the current studies, the lower abilities ...

Authors: Mengdan Zhang, Qian Li, Tingting Yang, Fei Meng, Xiaowei Lai, Lining Liang, Changpeng Li, Hao Sun, Jiaqi Sun and Hui Zheng

Erastin-induced ferroptosis is a regulator for the growth and function of human pancreatic islet-like cell clusters

Ferroptosis is a newly identified and novel form of cell death, which is characterized by an iron- and reactive oxygen species (ROS)-dependent manner. Potential utility of ferroptotic cell death has been recen...

Authors: Xing Yu Li and Po Sing Leung

Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance

Apparent Young’s modulus (AYM), which reflects the fundamental mechanical property of live cells measured by atomic force microscopy and is determined by substrate stiffness regulated cytoskeletal organization...

Authors: Anqi Guo, Bingjie Wang, Cheng Lyu, Wenjing Li, Yaozu Wu, Lu Zhu, Ran Bi, Chenyu Huang, Jiao Jiao Li and Yanan Du

Intestinal epithelial plasticity and regeneration via cell dedifferentiation

The intestinal epithelium possesses a great capacity of self-renewal under normal homeostatic conditions and of regeneration upon damages. The renewal and regenerative processes are driven by intestinal stem c...

Authors: Yuan Liu and Ye-Guang Chen

Comparative characterization and osteogenic / adipogenic differentiation of mesenchymal stem cells derived from male rat hair follicles and bone marrow

Clinical applications of cell therapy and tissue regeneration under different conditions need a multiplicity of adult stem cell sources. Up to date, little is available on the comparative isolation, characteri...

Authors: Abdel Kader A. Zaki, Tariq I. Almundarij and Faten A. M. Abo-Aziza

CRISPR/Cas: a potential gene-editing tool in the nervous system

The rapidly developmental RNA-guided CRISPR/Cas system is a powerful tool for RNA and DNA editing in a variety of cells from different species and makes a great contribution to gene function research, disease ...

Authors: Yanxia Gao, Kexin Gao and Hui Yang

Mapping a mammalian adult adrenal gland hierarchy across species by microwell-seq

Recently, single-cell RNA-seq technologies have been rapidly updated, leading to a revolution in biology. We previously developed Microwell-seq, a cost-effective and high-throughput single cell RNA sequencing(...

Authors: Shujing Lai, Lifeng Ma, Weigao E, Fang Ye, Haide Chen, Xiaoping Han and Guoji Guo

Comparison of Scanpy-based algorithms to remove the batch effect from single-cell RNA-seq data

With the development of single-cell RNA sequencing (scRNA-seq) technology, analysts need to integrate hundreds of thousands of cells with multiple experimental batches. It is becoming increasingly difficult fo...

Authors: Jiaqi Li, Chengxuan Yu, Lifeng Ma, Jingjing Wang and Guoji Guo

In vivo generation and regeneration of β cells in zebrafish

The pathological feature of diabetes, hyperglycemia, is a result of an inadequate number and/or function of insulin producing β cells. Replenishing functional β cells is a strategy to cure the disease. Althoug...

Authors: Bingyuan Yang, Brittney A. Covington and Wenbiao Chen

Capn3 depletion causes Chk1 and Wee1 accumulation and disrupts synchronization of cell cycle reentry during liver regeneration after partial hepatectomy

Recovery of liver mass to a healthy liver donor by compensatory regeneration after partial hepatectomy (PH) is a prerequisite for liver transplantation. Synchronized cell cycle reentry of the existing hepatocy...

Authors: Feng Chen, Delai Huang, Hui Shi, Ce Gao, Yingchun Wang and Jinrong Peng

Adipose tissue-derived mesenchymal stem cells and chitosan/poly (vinyl alcohol) nanofibrous scaffolds for cartilage tissue engineering

Osteoarthritis (OA) has been defined as a chronic inflammatory joint disease characterized by progressive articular cartilage degeneration. Recently growing interest in regenerative medicine, using cell therap...

Authors: Ghada Nour-Eldeen, Mazen Abdel-Rasheed, Amira M. EL-Rafei, Osama Azmy and Gehan T. El-Bassyouni

Generation of 3D human gastrointestinal organoids: principle and applications

The stomach and intestine are important organs for food digestion, nutrient absorption, immune protection and hormone production. Gastrointestinal diseases such as cancer and ulcer are big threats to human hea...

Authors: Mengxian Zhang, Yuan Liu and Ye-Guang Chen

Effectiveness of mesenchymal stem cell-conditioned medium in bone regeneration in animal and human models: a systematic review and meta-analysis

Given the limitations of current therapies for the reconstruction of bone defects, regenerative medicine has arisen as a new therapeutic strategy along with mesenchymal stem cells (MSCs), which, because of the...

Authors: Maria Paula Benavides-Castellanos, Nathaly Garzón-Orjuela and Itali Linero

Retrotransposons in pluripotent stem cells

Transposable elements constitute about half of the mammalian genome, and can be divided into two classes: the class I (retrotransposons) and the class II (DNA transposons). A few hundred types of retrotranspos...

Authors: Jingwen Wang, Junjiu Huang and Guang Shi

Autologous bone marrow mononuclear cell transplantation in patients with chronic traumatic brain injury- a clinical study

Chronic Traumatic Brain Injury (TBI) is one of the common causes of longterm disability worldwide. Cell transplantation has gained attention as a prospective therapeutic option for neurotraumatic disorders lik...

Authors: Alok K. Sharma, Hemangi M. Sane, Pooja P. Kulkarni, Nandini Gokulchandran, Hema Biju and Prerna B. Badhe

Dedifferentiation: the return road to repair the intestinal epithelium

In the March 5 issue of Cell Stem Cell, (Murata K et al. Cell Stem Cell. 26(377–390):e376 2020) reported that intestinal stem cell recovery after injury is principally through Ascl2-dependent dedifferentiation of...

Authors: Yuan Liu, Xiaochen Xiong and Ye-Guang Chen

Welcome to Cell Regeneration

Authors: Ye-Guang Chen and Ying Lou

Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish

We are using genetics to identify genes specifically involved in hearing regeneration. In a large-scale genetic screening, we identified mgat5a, a gene in the N-glycosylation biosynthesis pathway whose activity n...

Authors: Wuhong Pei, Sunny C. Huang, Lisha Xu, Kade Pettie, María Laura Ceci, Mario Sánchez, Miguel L. Allende and Shawn M. Burgess

Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency

Increasing evidence indicates that microRNAs (miRNAs), endogenous short non-coding RNAs 19–24 nucleotides in length, play key regulatory roles in various biological events at the post-transcriptional level. Em...

Authors: Young Jin Lee, Suresh Ramakrishna, Himanshu Chauhan, Won Sun Park, Seok-Ho Hong and Kye-Seong Kim

RNA-binding proteins in mouse male germline stem cells: a mammalian perspective

Adult stem cells that reside in particular types of tissues are responsible for tissue homeostasis and regeneration. Cellular functions of adult stem cells are intricately related to the gene expression progra...

Authors: Huayu Qi

Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo

Currently, direct conversion from somatic cells to neurons requires virus-mediated delivery of at least one transcriptional factor or a combination of several small-molecule compounds. Delivery of transcriptio...

Authors: Songwei He, Yiping Guo, Yixin Zhang, Yuan Li, Chengqian Feng, Xiang Li, Lilong Lin, Lin Guo, Haitao Wang, Chunhua Liu, Yi Zheng, Chuanming Luo, Qiang Liu, Fuhui Wang, Hao Sun, Lining Liang…

Stem cell therapy for amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the loss of motor neurons. Currently, no effective therapy is available to treat ALS, except for Riluzole, which has o...

Authors: Zhijuan Mao, Suming Zhang and Hong Chen

Reprogramming barriers and enhancers: strategies to enhance the efficiency and kinetics of induced pluripotency

Induced pluripotent stem cells are powerful tools for disease modeling, drug screening, and cell transplantation therapies. These cells can be generated directly from somatic cells by ectopic expression of def...

Authors: Behnam Ebrahimi

Regulatory functions and pathological relevance of the MECP2 3′UTR in the central nervous system

Methyl-CpG-binding protein 2 (MeCP2), encoded by the gene MECP2, is a transcriptional regulator and chromatin-remodeling protein, which is ubiquitously expressed and plays an essential role in the development and...

Authors: Heather McGowan and Zhiping P. Pang

Vascularization mediated by mesenchymal stem cells from bone marrow and adipose tissue: a comparison

Tissue-engineered constructs are promising to overcome shortage of organ donors and to reconstruct at least parts of injured or diseased tissues or organs. However, oxygen and nutrient supply are limiting fact...

Authors: Karoline Pill, Sandra Hofmann, Heinz Redl and Wolfgang Holnthoner

Stem cell aging in adult progeria

Aging is considered an irreversible biological process and also a major risk factor for a spectrum of geriatric diseases. Advanced age-related decline in physiological functions, such as neurodegeneration, dev...

Authors: Hoi-Hung Cheung, Duanqing Pei and Wai-Yee Chan

Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art

In the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous cell pop...

Authors: Eleni Oberbauer, Carolin Steffenhagen, Christoph Wurzer, Christian Gabriel, Heinz Redl and Susanne Wolbank

The interplay between metabolic homeostasis and neurodegeneration: insights into the neurometabolic nature of amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal, neurodegenerative disease that is characterized by the selective degeneration of upper motor neurons and lower spinal motor neurons, resulting in the progressive...

Authors: S. T. Ngo and F. J. Steyn

GATA2^−/− human ESCs undergo attenuated endothelial to hematopoietic transition and thereafter granulocyte commitment

Hematopoiesis is a progressive process collectively controlled by an elaborate network of transcription factors (TFs). Among these TFs, GATA2 has been implicated to be critical for regulating multiple steps of...

Authors: Ke Huang, Juan Du, Ning Ma, Jiajun Liu, Pengfei Wu, Xiaoya Dong, Minghui Meng, Wenqian Wang, Xin Chen, Xi Shi, Qianyu Chen, Zhongzhou Yang, Shubin Chen, Jian Zhang, Yuhang Li, Wei Li…

Wnt/β-catenin signaling in heart regeneration

The ability to repair damaged or lost tissues varies significantly among vertebrates. The regenerative ability of the heart is clinically very relevant, because adult teleost fish and amphibians can regenerate...

Authors: Gunes Ozhan and Gilbert Weidinger

Pharmacological manipulation of transcription factor protein-protein interactions: opportunities and obstacles

Much research on transcription factor biology and their genetic pathways has been undertaken over the last 30 years, especially in the field of developmental biology and cancer. Yet, very little is known about...

Authors: Frank Fontaine, Jeroen Overman and Mathias François

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

Endothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the body. Ets variant 2 (ETV2) is transiently expressed in both zebrafish and mice and is nece...

Authors: Anne G Lindgren, Matthew B Veldman and Shuo Lin

Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells

Interest in adipose-derived stem cells (ASCs) has increased in recent years due to their multi-linage differentiation capabilities. While much work has been done to optimize the differentiation media, few stud...

Authors: Mark Ahearne, Joanne Lysaght and Amy P Lynch

Generation of multi-gene knockout rabbits using the Cas9/gRNA system

The prokaryotic clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) is a simple, robust and efficient technique for gene targeting in model organisms such as zebrafish, mi...

Authors: Quanmei Yan, Quanjun Zhang, Huaqiang Yang, Qingjian Zou, Chengcheng Tang, Nana Fan and Liangxue Lai

Production of transgenic pigs over-expressing the antiviral gene Mx1

The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use o...

Authors: Quanmei Yan, Huaqiang Yang, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Nana Fan, Hongsheng Ouyang, Weiwang Gu and Liangxue Lai

Exploring the utility of organo-polyoxometalate hybrids to inhibit SOX transcription factors

SOX transcription factors constitute an attractive target class for intervention with small molecules as they play a prominent role in the field of regenerative biomedicine and cancer biology. However, rationa...

Authors: Kamesh Narasimhan, Kevin Micoine, Emmanuel Lacôte, Serge Thorimbert, Edwin Cheung, Bernold Hasenknopf and Ralf Jauch

Genetic reporter analysis reveals an expandable reservoir of OCT4+ cells in adult skin

The transcription factor Oct4 (Pou5f1) is a critical regulator of pluripotency in embryonic and induced pluripotent stem cells. Therefore, Oct4 expression might identify somatic stem cell populations with inheren...

Authors: Anne Limbourg, Sabine Schnabel, Vladimir J Lozanovski, L Christian Napp, Teng-Cheong Ha, Tobias Maetzig, Johann Bauersachs, Hassan Y Naim, Axel Schambach and Florian P Limbourg

Bioengineering of a human whole tooth: progress and challenge

A major challenge in stem cell-based bioengineering of an implantable human tooth is to identify appropriate sources of postnatal stem cells that are odontogenic competent as the epithelial component due to th...

Authors: Yanding Zhang and YiPing Chen

Role of Oct4 in the early embryo development

Oct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs) or their di...

Authors: Guangming Wu and Hans R Schöler

OCT4: A penetrant pluripotency inducer

Native OCT4 protein has the intrinsic ability of crossing cellular membranes to enter cells. This finding could revive efforts to induce pluripotency with proteins replacing nucleic acid-based approaches, and ...

Authors: Xuecong Wang and Ralf Jauch

A mitochondrial strategy for safeguarding the reprogrammed genome

Genomic aberrations induced by somatic cell reprogramming are a major drawback for future applications of this technology in regenerative medicine. A new study by Ji et al. published in Stem Cell Reports sugge...

Authors: Alessandro Prigione and James Adjaye

Aberrant DNA methylation reprogramming during induced pluripotent stem cell generation is dependent on the choice of reprogramming factors

The conversion of somatic cells into pluripotent stem cells via overexpression of reprogramming factors involves epigenetic remodeling. DNA methylation at a significant proportion of CpG sites in induced pluri...

Authors: Aline C Planello, Junfeng Ji, Vivek Sharma, Rajat Singhania, Faridah Mbabaali, Fabian Müller, Javier A Alfaro, Christoph Bock, Daniel D De Carvalho and Nizar N Batada

Generation of knockout rabbits using transcription activator-like effector nucleases

Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-spec...

Authors: Yu Wang, Nana Fan, Jun Song, Juan Zhong, Xiaogang Guo, Weihua Tian, Quanjun Zhang, Fenggong Cui, Li Li, Philip N Newsome, Jon Frampton, Miguel A Esteban and Liangxue Lai

Characterization of a novel cell penetrating peptide derived from human Oct4

Oct4 is a transcription factor that plays a major role for the preservation of the pluripotent state in embryonic stem cells as well as for efficient reprogramming of somatic cells to induced pluripotent stem ...

Authors: Eva Harreither, Hanna A Rydberg, Helene L Åmand, Vaibhav Jadhav, Lukas Fliedl, Christina Benda, Miguel A Esteban, Duanqing Pei, Nicole Borth, Regina Grillari-Voglauer, Oliver Hommerding, Frank Edenhofer, Bengt Nordén and Johannes Grillari

glbase: a framework for combining, analyzing and displaying heterogeneous genomic and high-throughput sequencing data

Genomic datasets and the tools to analyze them have proliferated at an astonishing rate. However, such tools are often poorly integrated with each other: each program typically produces its own custom output i...

Authors: Andrew Paul Hutchins, Ralf Jauch, Mateusz Dyla and Diego Miranda-Saavedra

Defining synthetic surfaces for human pluripotent stem cell culture

Human pluripotent stem cells (hPSCs) are able to self-renew indefinitely and to differentiate into all adult cell types. hPSCs therefore show potential for application to drug screening, disease modelling and ...

Authors: Jack W Lambshead, Laurence Meagher, Carmel O'Brien and Andrew L Laslett