Fig. 3
From: Mapping a mammalian adult adrenal gland hierarchy across species by microwell-seq
Comprehensive comparison of the BGI MGISEQ2000 and Illumina HiSeq platforms (a) We used BGI MGISEQ2000 and Illumina HiSeq sequencing platforms with a matched mouse adrenal gland single cell cDNA library. The BGI MGISEQ2000 and Illumina HiSeq platforms were compared in terms of cell number, total number of genes detected, mean gene number per cell, mean UMI counts per cell, and ratio of the reads mapped to the genome. b A bar chart of the detected gene number based on matched reads from the two sequencing platforms. c A line graph shows the gene number of the two sequencing platforms with respect to the matched UMIs. d The circle diagram summarizes the genes detected by the two platforms. We detected 23,136 genes and 22,476 genes from the sequence data generated by the BGI platform and the HiSeq platform, respectively. e The circle diagram summarizes the cell numbers detected by the two platforms. We detected 11,883 cells and 10,406 cells from the sequence data generated by the BGI platform and the HiSeq platform, respectively. f Comparison of the BGI MGISEQ2000 and Illumina HiSeq platform, Microwell-seq and 10× Genomics. Q30 is a value of sequencing accuracy, with the base misidentification < 0.1% during sequencing. A high Q30 value indicates high sequencing quality