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Cell Regeneration
Fig. 6 | Cell Regeneration

Fig. 6

From: Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance

Fig. 6

Maintenance of lineage specificity in HBs during in vitro culture on different substrate stiffness. a AYM measurement of parental HBs with the AFP-mCherry reporter using AFM. b Brightfield and immunostaining images of AFP for HBs cultured for 5 days on substrates with different stiffness. Scale bars = 100 μm. c PROX1 and AFP expression in HBs cultured for 5 days on substrates with different stiffness. d Growth curve of HBs over 3 passages on the 3.5 kPa substrate and coverslip. FACS analysis of PROX1 and AFP e in HBs cultured for 5 days on substrates with different stiffness, and f in 3 passages of HBs on the 3.5 kPa substrate and coverslip. g PROX1 and AFP expression in 3 passages of DE on the 3.5 kPa substrate and coverslip. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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