Fig. 3

Involvement of MAPK pathway in the induction of ferroptosis in ICCs. ICCs were exposed to 20 μM erastin for 24 h. The expression levels of unphosphorylated and phosphorylated of Erk, JNK and P38 were detected with images (a) and quantification (b) by Western blot analysis. c ICCs were challenged by 20 μM erastin with or without SP600125 (5 μM, 10 μM and 30 μM) for 24 h and the cell free supernatants were harvested for the measurement of LDH release. The data were compared with the non-treatment control group. (n = 3 per group; *p < 0.05, **p < 0.01, ***p < 0.001. All data are expressed as means ± SEM)