Organoid based personalized medicine: from bench to bedside

Cell Regeneration

Table 1 Comparison of the conditioned media requirements for the patient-derived organoids culture of respective cancer types

brain	head and neck	thyroid	breast	airway	ovary	prostate	bladder	kidney	esophagus	stomach	liver	pancreas	colon
B27	B27	B27	B27	B27	B27	B27	—	B27	B27	B27^a	B27	B27	B27
—	NACE	NACE	NACE	NACE	NACE	NACE	—	NACE	NACE	NACE	NACE	NACE	NACE
—	NICO	NICO	NICO	NICO	NICO	NICO	—	NICO	NICO	—	NICO	NICO	NICO
—	EGF	EGF	EGF	EGF	EGF	EGF	EGF	EGF	EGF	EGF	EGF	EGF	EGF
—	RSPO	RSPO	RSPO	—	RSPO	RSPO	—	—	RSPO	RSPO	RSPO	RSPO	RSPO
—	Noggin	Noggin	Noggin	—	Noggin	Noggin	—	—	Noggin	Noggin	—	Noggin	Noggin
—	A83-01	A83-01	A83-01	A83-01	A83-01	A83-01	—	A83-01	A83-01	A83-01	A83-01	A83-01	A83-01
—	FGF10	—	FGF10	—	FGF10	FGF10	—	—	FGF10	FGF10	FGF10	FGF10	—
—	FGF2	gastrin	—	—	FGF2	FGF2	—	—	gastrin	gastrin	gastrin	gastrin	gastrin
—	—	SB202190	SB202190	—	SB202190	SB202190	—	—	SB202190	—	HGF	—	SB202190
N2	Forskolin	—	Neuregulin 1	—	—	DHT	DHT	—	N2	—	N2	—	N2
human insulin	PGE2	—	Y27632	—	PGE2	Y27632	Y27632	—	—	—	forskolin	PGE2	PGE2
2-mercaptoethanol	CHIR99021	Wnt3a	—	—	—	—	—	—	Wnt3a	Wnt3a	Wnt3a	Wnt3a	—
(Jaffe et al. 2019)	(Driehuis et al. 2019b)	(Neal et al. 2018)	(Sachs et al. 2018)	(Sachs et al. 2019)	(Hill et al. 2018)	(Chua et al. 2014)	(Lee et al. 2018)	(Schutgens et al. 2019)	(Sato et al. 2011)	(Yan et al. 2018)	(Nuciforo et al. 2018)	(Driehuis et al. 2019c)	(Sato et al. 2011)

^aB27 without vitamin A is needed for culture of stomach organoid
Abbreviation: NACE N-acetylcysteine, NICO Nicotinamide, RSPO R-spondin-1, PGE2 Prostaglandin E2, DHT Dihydrotestosterone