Fig. 6

High RhoA/Rock/Jak1/Gsk3β activity is inversely correlated with Wnt/β-catenin signaling activity in the BMMSCs from elderly subjects. a-e, h-l Immunofluorescence images co-stained Nestin with p-Rock2, p-Tyr1022-Jak1, p-Tyr216-Gsk3β, p-S33-β-catenin (p-β-cat), or non-p-Ser45-β-catenin (a-β-cat) in bone marrow smears from either elderly men (n = 4) with femoral neck fractures and type II osteoporosis or young males (n = 3) with traumatic fractures and normal bone volumes, and in the proximal tibia sections of 5- or 10-month-old mice. f, m The percentages of p-Rock2⁺, p-Tyr1022-Jak1⁺, p-Tyr216-Gsk3β⁺, p-β-cat⁺, or a-β-cat⁺ cells in Nestin⁺ cells. g Quantitative RT-PCR analyses for LEF1, Cyclin D1 (C.D1), AXIN2, and RUNX2 mRNA levels in the BMMSCs isolated form the elderly or young men. Mean ± SEM, ^**p < 0.01, Student’s t test