Fig. 1

Phase Separation of OCT4 Controls TAD Reorganization for Cell Fate Transitions. (A) TAD reorganization can be manipulated by artificial looping or boundary deletion, respectively. Manipulation of TAD reorganization in MEFs can significantly enhance reprogramming efficiency. (B) OCT4 is phase separated during reprogramming. IDR mutation disrupts OCT4 phase separation, which can be rescued by fusing the IDR of FUS behind OCT4 mutant. OCT4 mutants attenuate TAD reorganization, which can be restored by IDR fusion. (C) OCT4 mutants reduce reprogramming efficiency, which can be recovered by IDR fusion