Insights into the present and future of cartilage regeneration and joint repair

Evenbratt, H.; Andreasson, L.; Bicknell, V.; Brittberg, M.; Mobini, R.; Simonsson, S.

doi:10.1186/s13619-021-00104-5

Cell Regeneration

Table 1 Characterization of chondrocytes or chondroprogenitors

From: Insights into the present and future of cartilage regeneration and joint repair

Cell origin	qPCR	Immunostaining	Histology	FACS	Reference
ESCs	COL2, AGGRECAN, SOX9, SOX6, SOX5, COL9	COL2, SOX9	Safranin O, Hematoxylin and Eosin	SOX9	(Cheng et al., 2014)
ESCs	COL2, AGGRECAN, SOX9, SOX6, SOX5, CD44	COL2, SOX9		SOX9, CD44	(Oldershaw et al., 2010)
ESCs	COL2, AGGRECAN, SOX5	SOX9	Safranin O, Hematoxylin and Eosin	SOX9	(Wang et al., 2019)
MSCs	COL2, AGGRECAN, SOX9	COL2, chondroitin sulfate	Hematoxylin and Eosin, Alcian Blue		(Weissenberger et al., 2020)
MSCs	COL2, AGGRECAN, SOX9	COL2	Hematoxylin and Eosin, Alcian Blue, Safranin O	CD44	(Meng et al., 2016)
MSCs	COL2, AGGRECAN	COL2, AGGRECAN	Hematoxylin, Alcian Blue		(Lu et al., 2017)
iPSCs+ESCs	COL2, AGGRECAN, SOX9	COL2, SOX9, chondroitin sulfate	Safranin O, Alcian Blue van Gieson, Toluidine blue, Hematoxylin, and Eosin		(Lach et al., 2019)
iPSCs	COL2, SOX9, SOX6, SOX5	COL2, AGGRECAN, SOX9, SOX6, COL9, COMP		CD44, CD151	(Suchorska et al., 2017a)
iPSCs	COL2, AGGRECAN, SOX9, SOX6, COL9, COMP	COL2, AGGRECAN, SOX9, SOX6, COL9, COMP			(Suchorska et al., 2017b)
iPSCs	COL2, AGGRECAN	COL2, AGGRECAN	Safranin O		(Diederichs et al., 2019)
iPSCs	COL2, AGGRECAN, SOX9, COL9, COL11	COL2	Alcian Blue, Hematoxylin and Eosin		(Nejadnik et al., 2015)
iPSCs	COL2, AGGRECAN, SOX9	COL2	Safranin O, Alcian Blue van Gieson, Hematoxylin and Eosin		(Nguyen et al., 2017)
iPSCs	COL2, AGGRECAN, SOX9, SOX6, SOX5, LUBRICIN	COL2	Toluidine blue		(Rim et al., 2018)
iPSCs	COL2, AGGRECAN, COMP	COL2	Toluidine blue, Hematoxylin, and Eosin		(Wei et al., 2012)
iPSCs	COL2, AGGRECAN, SOX9	COL2	Safranin O, Hematoxylin	CD105, CD145, CD166, CD271	(Adkar et al., 2019)
iPSCs	COL2, AGGRECAN, SOX9	COL2, AGGRECAN	Alcian Blue, Toluidine blue		(Koyama et al., 2013)
Chondrocytes	COL2, AGGRECAN, SOX9		Alcian Blue van Gieson		(Enochson et al., 2014)
Chondrocytes		COL2	Safranin O, Alcian Blue van Gieson		(Tallheden et al., 2004)
Chondrocytes	COL2, AGGRECAN	COL2, AGGRECAN			(Naranda et al., 2017)
Chondrocytes	COL2, AGGRECAN, COMP		Toluidine blue, Hematoxylin, and Eosin		(Wei et al., 2012)

Published articles were reviewed to overview how different research groups characterize their chondrocytes or chondroprogenitors originating from differentiated ES, iPSCs, MSCs, or chondrocytes. This table shows targeted genes and proteins in qPCR and immunoassays, histological stainings used, and which antigens were targeted with FACS.

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