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Cell Regeneration

Table 1 Characterization of chondrocytes or chondroprogenitors

From: Insights into the present and future of cartilage regeneration and joint repair

Cell origin qPCR Immunostaining Histology FACS Reference
ESCs COL2, AGGRECAN, SOX9, SOX6, SOX5, COL9 COL2, SOX9 Safranin O, Hematoxylin and Eosin SOX9 (Cheng et al., 2014)
ESCs COL2, AGGRECAN, SOX9, SOX6, SOX5, CD44 COL2, SOX9   SOX9, CD44 (Oldershaw et al., 2010)
ESCs COL2, AGGRECAN, SOX5 SOX9 Safranin O, Hematoxylin and Eosin SOX9 (Wang et al., 2019)
MSCs COL2, AGGRECAN, SOX9 COL2, chondroitin sulfate Hematoxylin and Eosin, Alcian Blue   (Weissenberger et al., 2020)
MSCs COL2, AGGRECAN, SOX9 COL2 Hematoxylin and Eosin, Alcian Blue, Safranin O CD44 (Meng et al., 2016)
MSCs COL2, AGGRECAN COL2, AGGRECAN Hematoxylin, Alcian Blue   (Lu et al., 2017)
iPSCs+ESCs COL2, AGGRECAN, SOX9 COL2, SOX9, chondroitin sulfate Safranin O, Alcian Blue van Gieson, Toluidine blue, Hematoxylin, and Eosin   (Lach et al., 2019)
iPSCs COL2, SOX9, SOX6, SOX5 COL2, AGGRECAN, SOX9, SOX6, COL9, COMP   CD44, CD151 (Suchorska et al., 2017a)
iPSCs COL2, AGGRECAN, SOX9, SOX6, COL9, COMP COL2, AGGRECAN, SOX9, SOX6, COL9, COMP    (Suchorska et al., 2017b)
iPSCs COL2, AGGRECAN COL2, AGGRECAN Safranin O   (Diederichs et al., 2019)
iPSCs COL2, AGGRECAN, SOX9, COL9, COL11 COL2 Alcian Blue, Hematoxylin and Eosin   (Nejadnik et al., 2015)
iPSCs COL2, AGGRECAN, SOX9 COL2 Safranin O, Alcian Blue van Gieson, Hematoxylin and Eosin   (Nguyen et al., 2017)
iPSCs COL2, AGGRECAN, SOX9, SOX6, SOX5, LUBRICIN COL2 Toluidine blue   (Rim et al., 2018)
iPSCs COL2, AGGRECAN, COMP COL2 Toluidine blue, Hematoxylin, and Eosin   (Wei et al., 2012)
iPSCs COL2, AGGRECAN, SOX9 COL2 Safranin O, Hematoxylin CD105, CD145, CD166, CD271 (Adkar et al., 2019)
iPSCs COL2, AGGRECAN, SOX9 COL2, AGGRECAN Alcian Blue, Toluidine blue   (Koyama et al., 2013)
Chondrocytes COL2, AGGRECAN, SOX9   Alcian Blue van Gieson   (Enochson et al., 2014)
Chondrocytes   COL2 Safranin O, Alcian Blue van Gieson   (Tallheden et al., 2004)
Chondrocytes COL2, AGGRECAN COL2, AGGRECAN    (Naranda et al., 2017)
Chondrocytes COL2, AGGRECAN, COMP   Toluidine blue, Hematoxylin, and Eosin   (Wei et al., 2012)
  1. Published articles were reviewed to overview how different research groups characterize their chondrocytes or chondroprogenitors originating from differentiated ES, iPSCs, MSCs, or chondrocytes. This table shows targeted genes and proteins in qPCR and immunoassays, histological stainings used, and which antigens were targeted with FACS.
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