Fig. 7

Chchd10 KO mice had impaired cold tolerance and reduced level of UCP1 in subcutaneous iWAT after cold treatment. A Rectal core temperature of WT and Chchd10^KO mice in the first 24 h after challenged by cold exposure (6 °C). Food was provided during cold-exposure. * p < 0.05, n = 6. B Quantitative PCR detection of the relative mRNA levels of Ucp1, Pgc1α and Pparγ in iWAT of WT and Chchd10^KO mice after 7 days of cold treatment. * p < 0.05, n = 3. C Western blot showing relative protein levels of UCP1, AP2, PPARγ in iWAT of WT and Chchd10^KO mice after cold treatment. D Western blot showing relative abundance of mitochondrial Complex I-V proteins in iWAT of WT and Chchd10^KO mice after cold treatment