Fig. 1

miR-378 regulates glycolytic metabolism of myofibers in mice. A Frozen sections of soleus muscle from WT and miR-378 TG mice were immunostained for different myosin heavy chain isoforms (n = 8 mice per group). B Quantification of immunofluorescence data shown in (A) expressed as the mean percentage of total muscle fibers. C Representative histochemical staining of α-GPDH (left) and SDH (right) enzymatic activity in gastrocnemius muscle from WT and TG mice (n = 8 mice per group). Scale bar, 20 μm. D Expression of HK2 and PFK1 represented glycolytic metabolic genes in soleus muscle from the indicated genotypes (n = 8 mice per group). E Tibialis anterior muscle lactate content in running time-matched mice (n = 8 mice per group). F Tibialis anterior muscle glycogen content in running time-matched mice (n = 8 mice per group) G Running time of WT and miR-378 TG mice at exhaustion (n = 8 mice per group). H Peak tetanic force of EDL muscle in the miR-378 TG and wild-type mice (n = 8 mice per group)