Fig. 4

AKT1 is one of the targets of miR-378 in skeletal muscle. A Protein levels of AKT1 in TA muscle from WT and the miR-378 TG mice were measured by western blot. GAPDH was used as a loading control. B Protein levels of AKT1, p-FoxO1, and FoxO1 in TA muscle intramuscularly injected with adenovirus expressing AKT1 (Ad-AKT1) of the miR-378 TG mice and WT littermates, determined by western blot. Adenovirus solely expressing EGFP (Ad-GFP) served as control. GAPDH was used as a loading control. C-F The expression of Myh2 (C), Myh4 (D), HK2 (E), and Pax7 (F) in WT and miR-378 TG mice intramuscularly injected with Ad-AKT1 as described in panel (B) (n = 3 mice per group)