Fig. 1

The growth of hGCSs but not hNSCs is inhibited by IFN-β. a A schematic representation showing hGCSs treated with basic media containing 10 ng/mL fibroblast growth factor (FGF), and different concentrations of IFN-β: 0, 0.625, 1.25, 2.5, 5, and 11 ng/mL. b The sphere-like hGCSs were inhibited, and more adherent hGCSs were observed after the IFN-β treatment. c IFN-β treatment resulted in smaller single sphere-like cells than control cells, and the sizes of the cell spheres are concentration-dependent within a certain range of IFN-β concentrations. d Coverage rate of adherent hGCSs decreased significantly with 5 ng/mL and 11 ng/mL IFN-β treatments. e Schematic representations of hGCSs treated with high concentrations of IFN-β. f High-concentration IFN-β also inhibits hGCS growth. g The coverage rate of hGCSs decreased after treatment with 11 ng/mL, 33 ng/mL, and 100 ng/mL IFN-β. h Schematic representation of hNSCs treated with high-concentration IFN-β. i High-concentration IFN-β does not inhibit hNSC growth. j The coverage rate of hNSCs does not decrease following treatment with 11 ng/mL, 33 ng/mL, and 100 ng/mL IFN-β. Quantitative data measured using ImageJ. Data are presented as the mean ± SD. Student’s t-test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001