Fig. 2

IFN-β treatment resulted in decreased numbers of Ki67-, S100-β-, and Sox2-positive cells. a Representative images of hGCSs immunostained for Ki67, S100-β, and Sox2, with DAPI nuclear stain. Three groups, control, 11 ng/mL, and 33 ng/mL IFN-β treatment groups, were collected after 6 days of treatment (scale bar, 50 μm; white frames indicate the next part of the figure). b Representative images from (A), at higher magnification, using the same three groups: control, 11 ng/mL, and 33 ng/mL IFN-β treatment groups. c Quantitative analysis of Ki67, S100-β, Sox2, and DAPI nuclear stain in hGCSs after IFN-β stimulation, as measured by ImageJ. Data are presented as the mean ± SD. Student’s t-test. ∗p < 0.05, ∗∗∗p < 0.001