Fig. 1
CTCF-K20 is acetylated by CBP. (A) Detection of the interaction between CTCF-Flag and the lysine-acetylated protein in HEK293T cells treated with TSA (10 μM) for 12 h. Membranes were immunoblotted with anti-Flag and anti-acetyl-lysine (Lys-Ac) antibodies after Flag co-IP experiments. (B) Detection of the interaction between Flag-tagged CTCF or the CTCF-K20R mutant and the lysine-acetylated protein in HEK293T cells. Membranes were immunoblotted with anti-Flag and anti-acetyl-lysine (Lys-Ac) antibodies after Flag co-IP experiments. (C) Western blots showing CTCF, CTCF-K20ac and β-ACTIN levels in control shRNA- and CTCF shRNA-depleted HEK293T cells. (D) Detection of the interaction between Flag-tagged CTCF or the CTCF-K20R mutant and CTCF-K20ac in HEK293T cells. Membranes were immunoblotted with anti-FLAG, anti-CTCF and anti-CTCF-K20ac antibodies after Flag co-IP experiments. (E) Western blots showing CTCF, CTCF-K20ac and β-ACTIN levels in HeLa-S3 cells treated with TSA (10 μM) and NAM (5 mM) for 12 h. (F) Western blots showing CTCF, CTCF-K20ac, Flag-tagged acetyltransferases and β-ACTIN levels in HeLa-S3 cells transfected with different constructs of Flag-tagged acetyltransferases. (G) Western blots showing CTCF, CTCF-K20ac and β-ACTIN levels in HEK293T cells treated with either the dimethyl sulfoxide (DMSO) control or the CBP inhibitor C646. (H) Detection of the interaction between CTCF and CBP using endogenous co-IP experiments. Membranes were immunoblotted with anti-CTCF and anti-CBP antibodies