Fig. 3
From: A mouse model of vitiligo based on endogenous auto-reactive CD8 + T cell targeting skin melanocyte
Whole-mount immunofluorescence staining to evaluate vitiligo induction efficiency. a Schematic diagram of the protocol for whole-mount immunofluorescent staining to detect melanocytes and CD8 + T cells in tail skin epidermis. b Representative whole-mount immunofluorescent images of epidermis separated by 20 mM EDTA or 1 × dispase. Scale bars, 100 µm. c Representative whole-mount immunofluorescent images, converted digital spots, scatter plots, and smoothed density plots showing the distribution pattern of melanocytes and CD8 + T cells on Day 33 after vitiligo induction. Scale bars, 500 µm. d Top: Representative whole-mount immunofluorescent images of melanocytes and CD8 + T cells on Day 19, 26, and 33 after vitiligo induction. Bottom: Scatter plots showing the distribution pattern of CD8 + T cells on the indicating time. Scale bars, 500 µm. e Quantification of T cell cluster size on Day 19, 26, and 33 after vitiligo induction. n = 8, 9, 7 mice for Day 19, 26, and 33, respectively