Fig. 1
From: Glioma stem cells and neural stem cells respond differently to BMP4 signaling
Bone morphogenic protein 4 (BMP4) inhibits the growth of human glioma stem cells (hGSCs) and changes the body size of human neural stem cells (hNSCs). a Schematic representation showing hGSCs treated with 0 ng/mL (Control) or 20 ng/mL BMP4 for 48 h. Left: hGSCs; right: hNSCs. The yellow box represents the source area of the magnified image. b Schematic representation showing hGSCs treated with 0 ng/mL (Control) or 20 ng/mL BMP4 for 96 h. Left: hGSCs; right: hNSCs. The yellow box represents the source area of the magnified image. c Quantitative analysis of hGSC sphere numbers measured using ImageJ. d Coverage areas of single hGSC spheres were calculated. e The body size of both hGSCs and hNSCs was observed and measured using ImageJ (× 4, × 10, and × 20 magnification). Scale bar, 50 μm. Data are presented as means ± SD. Student’s t-test. n.s., not significant; ∗∗∗p < 0.001