Fig. 6
From: Glioma stem cells and neural stem cells respond differently to BMP4 signaling
Continuous BMP4 treatment can strength the inhibition to hGSCs quantity and size, and is different from hNSCs. a Flowsheet of repeated exposure to BMP4 treatment and photographing time points. The effect of BMP4 continuous pressure was analyzed by the strategy of generation and repeated exposure. b Representative hGSCs pictures after BMP4 continuous pressure at re passage and treatment for 2 days. 0 ng/mL (Control), 20 ng/mL BMP4 (black frame indicates the second row enlarged images source). c Representative hNSCs pictures after BMP4 continuous pressure at re passage and treatment for 2 days. 0 ng/mL (Control), 20 ng/mL BMP4 (black frame indicates the second row enlarged images source). d Representative hGSCs pictures after BMP4 continuous pressure at re passage and treatment for 8 days. 0 ng/mL (Control), 20 ng/mL BMP4 (black frame indicates the third row enlarged images source). e-f Quantitative statistics of hGSCs after BMP4 repeated exposure, including number of hGSCs sphere (e) and Cell size (f) of remaining surviving clones/cells measuring by ImageJ. Scale bar, 50 μm. Data are presented as means ± SD. Student’sA t-test. ∗∗p < 0.01, ∗∗∗p < 0.001