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Cell Regeneration
Fig. 3 | Cell Regeneration

Fig. 3

From: Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism

Fig. 3

Live imaging of mitochondrial transport after axon injury and ischemia. Schematics (A, D) and representative kymographs (B, E) and quantifications (C, F) showing the bi-directional axonal mitochondrial transport after axotomy injury (A-C) or upon ischemia-reperfusion (D-F). Cortical neurons were infected with lentivirus encoding GFP-Mito, followed by live imaging at DIV14 before or post axotomy (B, C) or OGD-R treatment (E, F). Images were captured for 30 frames with 5-s intervals. In kymographs, vertical lines represent stationary mitochondria; oblique lines or curves to the right indicate anterograde transport toward distal terminals, whereas those to the left denote retrograde transport toward the cell body. Data were quantified from n = 27–37 microgrooves from more than 3 chambers per condition in three independent experiments. Data were expressed as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparisons test. Scale bars, 10 μm

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