Fig. 2From: PHDs-seq: a large-scale phenotypic screening method for drug discovery through parallel multi-readout quantificationSystematic evaluation of PHDs-seq. AÂ Schematic diagram for KF-adipocyte reprogramming. BÂ Clustering analysis of PHDs-seq data from positive and negative samples. Posi: keloid fibroblasts cultured in AD medium; KF: keloid fibroblasts cultured in KF medium; D5: day5; D8: day8; _1 and _2 represent two biological replicates. CÂ Pearson correlation analysis of PHDs-seq data from positive and negative samples. DÂ Validation of probe performance using redundant probe pairs for a single gene in PHDs-seq. EÂ Validation of consistency between biological replicates in PHDs-seq. F-G Comparison between PHDs-seq performance and qPCR assays in quantifying relative mRNA levels in samples collected at D5 (F)Â and D8 (G)Back to article page