Articles

Aberrant DNA methylation reprogramming during induced pluripotent stem cell generation is dependent on the choice of reprogramming factors

The conversion of somatic cells into pluripotent stem cells via overexpression of reprogramming factors involves epigenetic remodeling. DNA methylation at a significant proportion of CpG sites in induced pluri...

Authors: Aline C Planello, Junfeng Ji, Vivek Sharma, Rajat Singhania, Faridah Mbabaali, Fabian Müller, Javier A Alfaro, Christoph Bock, Daniel D De Carvalho and Nizar N Batada

Generation of knockout rabbits using transcription activator-like effector nucleases

Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-spec...

Authors: Yu Wang, Nana Fan, Jun Song, Juan Zhong, Xiaogang Guo, Weihua Tian, Quanjun Zhang, Fenggong Cui, Li Li, Philip N Newsome, Jon Frampton, Miguel A Esteban and Liangxue Lai

Characterization of a novel cell penetrating peptide derived from human Oct4

Oct4 is a transcription factor that plays a major role for the preservation of the pluripotent state in embryonic stem cells as well as for efficient reprogramming of somatic cells to induced pluripotent stem ...

Authors: Eva Harreither, Hanna A Rydberg, Helene L Åmand, Vaibhav Jadhav, Lukas Fliedl, Christina Benda, Miguel A Esteban, Duanqing Pei, Nicole Borth, Regina Grillari-Voglauer, Oliver Hommerding, Frank Edenhofer, Bengt Nordén and Johannes Grillari

glbase: a framework for combining, analyzing and displaying heterogeneous genomic and high-throughput sequencing data

Genomic datasets and the tools to analyze them have proliferated at an astonishing rate. However, such tools are often poorly integrated with each other: each program typically produces its own custom output i...

Authors: Andrew Paul Hutchins, Ralf Jauch, Mateusz Dyla and Diego Miranda-Saavedra

Defining synthetic surfaces for human pluripotent stem cell culture

Human pluripotent stem cells (hPSCs) are able to self-renew indefinitely and to differentiate into all adult cell types. hPSCs therefore show potential for application to drug screening, disease modelling and ...

Authors: Jack W Lambshead, Laurence Meagher, Carmel O'Brien and Andrew L Laslett

Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

Tooth is vital not only for a good smile, but also good health. Yet, we lose tooth regularly due to accidents or diseases. An ideal solution to this problem is to regenerate tooth with patients’ own cells. Her...

Authors: Jinglei Cai, Yanmei Zhang, Pengfei Liu, Shubin Chen, Xuan Wu, Yuhua Sun, Ang Li, Ke Huang, Rongping Luo, Lihui Wang, Ying Liu, Ting Zhou, Shicheng Wei, Guangjin Pan and Duanqing Pei

Targeted genome engineering in human induced pluripotent stem cells by penetrating TALENs

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) have been successfully used to knock out endogenous genes in stem cell research. However, the deficiencies of current g...

Authors: Renli Ru, Yongchao Yao, Songlin Yu, Benpeng Yin, Wanwan Xu, Siting Zhao, Li Qin and Xiaoping Chen

Cohesin complexes with a potential to link mammalian meiosis to cancer

Among multiple genes aberrantly activated in cancers, invariably, there is a group related to the capacity of cell to self-renewal. Some of these genes are related to the normal process of development, includi...

Authors: Alexander Strunnikov

SNX16 negatively regulates the migration and tumorigenesis of MCF-7 cells

Sorting nexins are a large family of proteins that are associated with various components of the endosome system and they play many roles in processes such as endocytosis, intracellular protein trafficking and...

Authors: Leilei Zhang, Dajiang Qin, Chunfang Hao, Xiaodong Shu and Duanqing Pei

The involvement of interleukin-22 in the expression of pancreatic beta cell regenerative Reg genes

In Type 1 diabetes, the insulin-producing β-cells within the pancreatic islets of Langerhans are destroyed. We showed previously that immunotherapy with Bacillus Calmette-Guerin (BCG) or complete Freund’s adju...

Authors: Thomas Hill, Olga Krougly, Enayat Nikoopour, Stacey Bellemore, Edwin Lee-Chan, Lynette A Fouser, David J Hill and Bhagirath Singh

Generation of functional organs from stem cells

We are now well entering the exciting era of stem cells. Potential stem cell therapy holds great promise for the treatment of many diseases such as stroke, traumatic brain injury, Alzheimer’s disease, Parkinso...

Authors: Yunying Liu, Ru Yang, Zuping He and Wei-Qiang Gao

Return to the hematopoietic stem cell origin

Studying embryonic hematopoiesis is complicated by diversity of its locations in the constantly changing anatomy and by the mobility of blood cell precursors. Embryonic hematopoietic progenitors are identified...

Authors: Igor M Samokhvalov

Using mouse models to study function of transcriptional factors in T cell development

Laboratory mice have widely been used as tools for basic biological research and models for studying human diseases. With the advances of genetic engineering and conditional knockout (CKO) mice, we now underst...

Authors: Peng Li, Yiren Xiao, Zhixin Liu and Pentao Liu

Pluripotency and its layers of complexity

Pluripotency is depicted by a self-renewing state that can competently differentiate to form the three germ layers. Different stages of early murine development can be captured on a petri dish, delineating a s...

Authors: Jolene Ooi and Pentao Liu

A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro

Human embryonic stem cells (hESCs) represent a tremendous resource for cell therapies and the study of human development; however to maintain their undifferentiated state in vitro they routinely require the use o...

Authors: Fiona C Lewis, Nicholas Bryan and John A Hunt

Progress and bottleneck in induced pluripotency

With their capability to undergo unlimited self-renewal and to differentiate into all cell types in the body, induced pluripotent stem cells (iPSCs), reprogrammed from somatic cells of individual patients with...

Authors: Zhen-Ning Zhang and Yang Xu

SNX17 regulates Notch pathway and pancreas development through the retromer-dependent recycling of Jag1

Notch is one of the most important signaling pathways involved in cell fate determination. Activation of the Notch pathway requires the binding of a membrane-bound ligand to the Notch receptor in the adjacent ...

Authors: Wenguang Yin, Dapeng Liu, Nian Liu, Liangliang Xu, Song Li, Shuo Lin, Xiaodong Shu and Duanqing Pei

Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells

Cell-based therapies show promise in repairing cardiac tissue and improving contractile performance following a myocardial infarction. Despite this, ischemia-induced death of transplanted cells remains a major...

Authors: Curtis C Hughey, Maria P Alfaro, Darrell D Belke, Jeffery N Rottman, Pampee P Young, David H Wasserman and Jane Shearer

Differentiation of human mesenchymal stem cell spheroids under microgravity conditions

To develop and characterize a novel cell culture method for the generation of undifferentiated and differentiated human mesenchymal stem cell 3D structures, we utilized the RWV system with a gelatin-based scaf...

Authors: Wolfgang H Cerwinka, Starlette M Sharp, Barbara D Boyan, Haiyen E Zhau, Leland W K Chung and Clayton Yates

Cell regeneration- bridging discoveries in applied and basic research

Authors: Duanqing Pei